DAPI and cluster

Fission yeast cells undergo sexual differentiation under conditions of nutritional stress, especially nitrogen starvation. Haploid cells of opposite mating types mate and fuse to form a diploid cell, which immediately undergoes meiosis and sporulation. We are using DNA microarrays to study the transcriptional program of sexual differentiation. We have started by analysing diploid cells undergoing meiosis and sporulation (see below). We are currently studying the first stages of sexual differentiation (response to nitrogen starvation and pheromone signalling) and systematically deleting transcription factors that may have a meiotic role.

Mata J, Lyne R, Burns G, and Bähler J (2002). The transcriptional program of meiosis and sporulation in fission yeast.
Nature Genet. 32:143-147.
A reprint is available as a PDF.

Gene expression viewer:
To check the expression value of your favourite gene use the search box below:
Use commas to separate multiple queries e.g. pat1,tea1.
Click on the gene name in the key (bottom of graph) to connect to GeneDB.

Experiment [How to cite these data] [Help]
Gene name
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Supplementary Tables:

  • All tables: All of the tables below in a single file (pdf).
  • Table A: Previously known genes expressed in meiosis and/or with a meiotic function.
  • Table B: Genes induced in response to starvation/pheromone (transient).
  • Table C: Genes induced in response to starvation/pheromone (continuous).
  • Table D: Genes induced in response to nitrogen starvation (delayed).
  • Table E: Early genes.
  • Table F: Middle genes.
  • Table G: Late genes.
  • Table H: Potential regulatory motifs.
  • Table I: Known and potential transcriptional regulators induced during meiosis and sporulation.
  • Table J: Core meiotic transcriptome conserved in fission and budding yeasts.

Complete Data Sets:

Descriptions of the experiments are available here.

  • pat1 diploid meiosis: average of four independent experiments:
    Download data (ftp)
  • pat1 diploid meiosis: separate results for four independent experiments:
    Download data (ftp)
  • pat1+ diploid: non-sporulating control (no nitrogen added at temperature-shift):
    Download data (ftp)
  • pat1+ diploid: non-sporulating control (nitrogen added at temperature-shift):
    Download data (ftp)
  • Wild type diploid meiosis:
    Download data (ftp)
  • Comparison of atf21 and atf31 mutants to wild type (h90 meiosis):
    Download data (ftp)

All raw data are available under accession numbers E-SNGR-2 to E-SNGR-7 from ArrayExpress.

Links:


Mata J and Bähler J (2006). Global roles of Ste11p, cell type, and pheromone in the control of gene expression during early sexual differentiation in fission yeast.
Proc. Natl. Acad. Sci. 103:15517-15522.
A reprint is available as a PDF.

Gene expression viewer:
To check the expression value of your favourite gene use the search box below:
Use commas to separate multiple queries e.g. pat1,tea1.
Click on the gene name in the key (bottom of graph) to connect to GeneDB.

Experiment [How to cite these data] [Help]
Gene name
match type scale type or as
Scale auto or set manually to min= and max=

Supplementary Tables:

Complete Data Sets:

Descriptions of the experiments are available here.

  • Nitrogen starvation time courses normalised to 0 hours (h+, h-, h90 fus1 and h90 ste11Δ)
    Download data (ftp)
  • Nitrogen starvation time courses normalised to h90fus1 at time 0 hours (h+, h-, h90 fus1 and h90 ste11Δ)
    Download data (ftp)
  • Comparisons between h90 ste11Δ and h90 fus1 after nitrogen starvation
    Download data (ftp)
  • Ste11p overproduction
    Download data (ftp)
  • Time course after pheromone treatment
    Download data (ftp)

All raw data are available under accession number E-TABM-139 from ArrayExpress.

Mata J, Wilbrey A and Bähler J (2007). Transcriptional regulatory network for sexual differentiation in fission yeast.
Genome Biol. 8(10):R217.
A reprint is available as a PDF

Additional Files:

  • Additional File 1: Complete normalized dataset for mei4Δ pat1 and pat1 time courses.
  • Additional File 2: Complete normalized dataset for rep1Δ pat1 and pat1 time courses.
  • Additional File 3: Complete normalized dataset for rsv1Δ and rsv2Δ experiments.
  • Additional File 4: Complete normalized dataset for Atf21p, Atf31p, Atf21p/Atf31p and Mei4p overexpression experiments.
  • Additional File 5 (doc): Table 1: Potential regulatory motifs in the promoters of Rsv1p-regulated genes. Table 2: Effects of rep1Δ on meiotic transcription: comparison with published data. Table 3: Effects of mei4Δ on meiotic transcription; comparison with published data.
  • Additional File 6 (xls): Middle genes classified into three groups according to the effect of mei4Δ and Mei4p overexpression on their expression.
  • Additional File 7 (xls): Genes induced by Atf21p, Atf31p and Atf21p/Atf31p overexpression and genes induced by Atf21p/Atf31p overexpression that are also reduced in atf21Δ meiotic cells.
  • Additional File 8 (xls): Genes induced by Mei4p overexpression, classified according to their expression profiles.
  • Additional File 9 (xls): Mei4p-dependent and Mei4p-independent middle genes based on the mei4Δ time course experiment.
  • Additional File 10 (xls): Complete list of potential Rsv1p targets.
  • Additional File 11 (xls): Complete list of potential Rsv2p targets.
  • Additional File 12 (xls): Rep1p-dependent and Rep1p-independent early genes based on the rep1Δ time course experiment.

All raw data are available under accession number E-TABM-298, E-TABM-299, E-TABM-300, E-TABM-301 from ArrayExpress.